Background and Objectives: Nursing is a non-stop job 24 hours a day, because patient caring is a full time task. The purpose of this study was to compare work-family conflict and burnout in nurses with and without rotating shifts.

Material and Methods: This was a causal-comparative study. The population of this study consisted of nurses in Tabriz hospitals in 2017 and the sample size for this study included 160 individuals selected randomly based on multi-stage cluster for each group. To collect data, Terez Work-Family Conflict Questionnaire (2010) and Maslach (1988) job burnout were applied. Data were analyzed by SPSS 21 using multivariate analysis of variance.

Results: The results showed that nurses with constant work shift reported less work-family conflict, family-work conflict, emotional exhaustion, depersonalization, personal failure and burnout than nurses with rotating shift work, and these differences were significant at P<0.01 level.

Conclusion: The results of this study showed that rotating nursing system causes problems in the family and nursing performance. This could have significant impact in policy-making for nursers’ health.